An ecophysiological experiment was conducted to examine the biochemical effects of acidified seawater containing elevated concentration of CO2 (CCO2 0.08, 0.20, 0.50 and 1.00%) on the copepod Centropages tenuiremis. AchE, ATPase, SOD, GPx, GST, GSH level and GSH/GSSG ratio of the copepod were analyzed. The results showed that elevated CCO2 and the duration of culture time significantly influenced several biochemical indices in C. tenuiremis (ATPase, GPx, GST, GSH and SOD). Furthermore, the principal component analysis results indicated that 72.32% of the overall variance was explained by the first three principal components (GPx, SOD and GSH). Changes in GPx and GSH levels may play a significant role in the antioxidant defense of copepods against seawater acidification. The long-term response of copepods to seawater acidification and the synergistic effects of acidification with other environmental factors, such as temperature, salinity and trace metal need further investigation.
Zhang D., Li S., Wang G., Guo D., Xing K., & Zhang S., 2012. Biochemical responses of the copepod Centropages tenuiremis to CO2-driven acidified seawater. Water Science & Technology 65(1):30-37. Article (subscription reqired).